RealLine Trichomonas vaginalis / Gardnerella vaginalis

The RealLine Trichomonas vaginalis / Gardnerella vaginalis kit is intended for a detection and differentiation of Gardnerella vaginalis and Trichomonas vaginalis DNA in clinical samples by Real-Time PCR.

All components are lyophilized and ready-to use.

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Trichomonas vaginalis – a flagellate – causes trichomoniasis, a disease that causes vaginal infections in women and urethritis in men. The transmission of trichomoniasis runs through sexual intercourse and is one of the most common sexually transmitted diseases.
Gardnerella vaginalis is a facultatively anaerobic Gram-variable rod that can cause bacterial vaginosis in some women as a result of a disruption in the normal vaginal microflora.

Sample Material

  • Epithelial cells swabs
  • Semen
  • Prostatic fluid
  • Urine

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • HEX (535 – 554 nm) for Gardnerella vaginalis DNA
  • ROX (575 – 602 nm) for Trichomonas vaginalis DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.


In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, Bio-Rad cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH)

CFX96, iQ5 iCycler (Bio-Rad, USA)

DTprime, DTLite (DNA-Technology, Russia)

Our kits are compatible to other Real-Time Cyclers. Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD0477

Additional information

Additional information

RealLine Trichomonas vaginalis / Gardnerella vaginalis

Str, Fla