RealLine TBEV (FSME)

The RealLine TBEV kit is intended for a sensitive detection of TBEV RNA in clinical samples or from ticks by one-step real-time RT-PCR. The kits are designed for in vitro diagnostics and qualitative detection.

All components are lyophilized and ready-to use.

GLU – for General Lab Use

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The Tick-Borne Encephalitis Virus is endemic in an area ranging from northern China and Japan, through far-eastern Russia to Europe. TBEV is transmitted by the bite of infected ticks (Ixodes scapularis, Ixodes ricinus and Ixodes persulcatus), or (rarely) through the non-pasteurized milk of infected cows, sheeps and goats. TBE is a viral infectious disease involving the central nervous system (CNS). The disease is incurable once manifested, so there is no specific drug therapy for TBE.

Sample Material

  • Blood serum, plasma
  • Cerebrospinal fluid
  • Tick suspension sample

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for TBEV RNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.


In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH)

CFX96, iQ5 iCycler (Bio-Rad, USA)

DT96, DTLite (DNA-Technology, Russia)

Our kits are compatibel to other Real-Time cyclers. Please read the IFU properly and ask us for recommendations of device settings!

Additional information

Additional information


Str, Fla