RealLine TBEV (FSME)

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The RealLine TBEV kit is intended for a sensitive detection of TBEV RNA in clinical samples or from ticks by one-step real-time RT-PCR. The kits are designed for in vitro diagnostics and qualitative detection.

All components are lyophilized and ready-to use.

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Description

Description

The Tick-Borne Encephlitis Virus is endemic in an area ranging from northern China and Japan, through far-eastern Russia to Europe. TBEV is transmitted by the bite of infected ticks (Ixodes scapularis, Ixodes ricinus and Ixodes persulcatus), or (rarely) through the non-pasteurized milk of infected cows, sheeps and goats. TBE is a viral infectious disease involving the central nervous system (CNS). The disease is incurable once manifested, so there is no specific drug therapy for TBE.

Sample Material

  • Blood serum, plasma
  • Cerebrospinal fluid
  • Tick suspension sample

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for TBEV RNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Fla-format

For Fla-format 10 Tests of the lyophilized Mastermix is filled in one vial. Pipette 300 µl of the Recovery Solution into the vial, incubate at RT for 15 min and then pipette 25 µl into your tubes or plates which fit optimally to your cycler. Add 25 µl of DNA/RNA after the extraction procedure and run the program.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

Eco48 (PCRMax, GB) for Fla-format

Our kits can be used with other Real-Time cyclers from ABI, Roche, Agilent etc.
Please read the IFU properly and ask us for recommendations of device settings!

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Additional information

Additional information

RealLine TBEV FSME

Str, Fla

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