RealLine Ureaplasma urealyticum / Ureaplasma parvum

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The RealLine Ureaplasma urealyticum / Ureaplasma parvum kit is inteded for the detection and differentiation of Ureaplasma urealyticum and Ureaplasma parvum DNA from clinical samples by Real-Time PCR.

All components are lyophilized and ready-to use.

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Description

Description

Ureaplasma parvum is generally the most common species detected in various clinical specimens. Ureaplasma urealyticum is apparently more pathogenic in conditions such as male urethritis .
Ureaplasma urealyticum is part of the normal genital flory of both men and women. In about 70 % of sexually active humans it is found and is described to be associated with a number of diseases in humans, including non-specific urethritis (NSU), infertility, chorioamnionitis, stillbirth, premature birth and other.

Sample Material

  • Scrapings of epithelial cells
  • Urine
  • Semen
  • Prostatic juice

Detection channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • HEX (535 – 554 nm) for Ureaplasma urealyticum DNA
  • ROX (575 – 602 nm) for Ureaplasma parvum DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Fla-format

For Fla-format 10 Tests of the lyophilized Mastermix is filled in one vial. Pipette 300 µl of the Recovery Solution into the vial, incubate at RT for 15 min and then pipette 25 µl into your tubes or plates which fit optimally to your cycler. Add 25 µl of DNA/RNA after the extraction procedure and run the program.

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RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

Eco48 (PCRMax, GB) for Fla-format

Our kits can be used with other Real-Time cyclers from ABI, Roche, Agilent etc.
Please read the IFU properly and ask us for recommendations of device settings!

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Additional information

Additional information

RealLine Ureaplasma urealyticum / Ureaplasma parvum

Str, Fla

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