RealLine Treponema pallidum

The RealLine Treponema pallidum kit is intended for the detection of Treponema pallidum DNA from clinical specimen by Real-Time PCR.

All components are lyophilized and ready-to use.

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Description

Description

Treponema pallidum is a motile spirochaete that is generally acquired by close sexual contact, entering the host via breaches in squamous or columnar epithelium. T. pallidum is causing Syphilis – a sexually transmitted infection. The primary route of transmission is through sexual contact; it may also be transmitted from mother to fetus during pregnancy or at birth, resulting in congenital syphilis.

Sample Material

  • Scrapings of epithelial cells (urethral, cervical, vaginal)
  • Tissue fluid from erosive and ulcerative lesions of the skin and mucous membranes.

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for Treponema pallidum DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, Bio-Rad cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD1898

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Additional information

Additional information

RealLine Treponema pallidum

Str, Fla

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