RealLine Rickettsia species

The RealLine Rickettsia species kit is detecting the subtypes of known species of Rickettsia by Real-Time PCR.

RUO – Research Use Only



Rickettsia are endemic bacterias and found in many vectors like ticks, fleas, mites and lice and are spreaded in Mediterranean countries, Eastern Europe, the tropics and North America. These bacteria cause in humans diverse diseases with different clinical pictures, which are grouped together medically to the group of Rickettsioses. Examples include typhus (syn. Typhus exanthematicus), rickettsialpox, Brill-Zinsser disease, Boutonneuse fever (Mediterranean tick-borne fever) and Rocky Mountain spotted fever.  Rickettsiae thrive – like viruses -, as intracellular parasites
exclusively in living cells. In this way, they manage to escape the immune system of their hosts.

Sample Material

  • Blood serum, plasma
  • Cerebrospinal fluid
  • Tick suspension samples

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for Rickettsia species DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.


In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.


For Fla-format 10 Tests of the lyophilized Mastermix is filled in one vial. Pipette 300 µl of the Recovery Solution into the vial, incubate at RT for 15 min and then pipette 25 µl into your tubes or plates which fit optimally to your cycler. Add 25 µl of DNA/RNA after the extraction procedure and run the program.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

Eco48 (PCRMax, GB) for Fla-format

Our kits can be used with other Real-Time cyclers from ABI, Roche, Agilent etc.
Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD5391


IFU Fla-format - VBD5392

Additional information

Additional information

RealLine Rickettsia species RUO

Str, Fla