RealLine HIV quantitative

The RealLine HIV quantitative Kit is intended for a sensitive detection and quantification of RNA of the Human Immunodeficiency Virus (HIV) by Reverse Transcription and Real-Time PCR in one tube.

RUO – Research Use Only



The Human immunodeficiency Virus (HIV) is a member of the genus Lentivirus, part of the family of Retroviridae. This virus causes the acquired immunodeficiency syndrome (AIDS). From its discovery in 1981 AIDS killed more than 25 million people and is worldwide spread.

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for HIV cDNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.


In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH)

CFX96, iQ5 iCycler (Bio-Rad, USA)

DTprime, DTLite (DNA-Technology, Russia)

Our kits are compatible to other Real-Time Cyclers. Please read the IFU properly and ask us for recommendations of device settings!

IFU - VBD0195

Additional information

Additional information

RealLine HIV quantitative RUO