RealLine Chlamydia trachomatis / Neisseria gonorrhoeae

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The RealLine Chlamydia trachomatis / Neisseria gonorrhoeae kits are designed for a differential and sensitive detection of  the plasmid and genomic DNA of Chlamydia trachomatis and Neisseria gonorrhoeae DNA in clinical samples by Real-Time PCR. The kits are designed for qualitative diagnosis.

All components are lyophilized and ready-to use.

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Description

Description

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Untreated infections by Chlamydia trachomatis can cause serious reproductive and other health problems with both short-term and long-term consequences. In both sexes Chlamydia infection can cause proctitis, trachoma and infertility as well as prostatis, epidiymitis in men and cervictis, PID, ectopic pregnancy and acute/chronic pelvic pain in women.

Neisseria gonorrhoeae are gram-negative motile bacteria and the causative agent of gonorrhea, one of the most common sexually transferred disease. In women gonorrhea can lead to severe symptoms such as infertility and abortion. In men gonorrhoeae can lead to painful urethritis.

RealLine Chlamydia trachomatis / Neisseria gonorrhoeae kits are regularly tested by External Quality Assessment Panels, see Quality Assurance

Sample Material

  • Scrapings of epithelial cells (urethral, cervical, vaginal)
  • Semen
  • Prostatic fluid
  • Urine

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • HEX (535 – 554 nm) for Chlamydia trachomatis DNA
  • ROX (575 – 602 nm) for Neisseria gonorrhoeae DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Fla-format

For Fla-format 10 Tests of the lyophilized Mastermix is filled in one vial. Pipette 300 µl of the Recovery Solution into the vial, incubate at RT for 15 min and then pipette 25 µl into your tubes or plates which fit optimally to your cycler. Add 25 µl of DNA/RNA after the extraction procedure and run the program.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

Eco48 (PCRMax, GB) for Fla-format

Our kits can be used with other Real-Time cyclers from ABI, Roche, Agilent etc.
Please read the IFU properly and ask us for recommendations of device settings!

Additional information

Additional information

RealLine Chlamydia trachomatis / Neisseria gonorrhoeae

Str, Fla

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