RealLine HPV HCR Genotype

The RealLine HPV HCR Genotype kit is intended for the sensitive detection and differentiation of 12 common high risk Genotypes of the Human Papillomavirus HPV.

All components are lyophilized and ready-to use.



More than 100 different Human Papilloma Viruses HPV are detected nowadays and some of these types are more likely to develop cancer in human beings. Those are  referred as “high carcinogenic risk”  or “high risk types” HPV and they are known to cause the vast majority of cervical cancers which cause death in women with an annual incidence of around half a million and a mortality of almost 50 %. But the persistent infection has also been linked to cancer of the oropharynx, larynx, vulva, vagina, penis, and anus.

The RealLine HPV HCR Genotype kit is detecting and differentiating following HPV genotypes in 4 mastermixes:
16, 18, 21, 33, 35, 39, 45, 51, 52, 56, 58, and 59.

Sample Material

  • Scrapings of the epithelial cells
  • Biopsies

Detection Channels (please read the IFU for details)

  • FAM
  • HEX
  • ROX
  • Cy5

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.


In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Fla-format (procedure only for VBD8482 Kit, 4 mastermixes: each 11 Tubes)

For Fla-format 9 Tests of the lyophilized Mastermix is filled in one vial. Pipette 250 µl of the Recovery Solution into the vial, incubate at RT for 15 min and then pipette 25 µl into your tubes or plates which fit optimally to your cycler. Add 25 µl of DNA/RNA after the extraction procedure and run the program.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

Eco48 (PCRMax, GB) for Fla-format

Our kits can be used with other Real-Time cyclers from ABI, Roche, Agilent etc.
Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD8479


IFU Fla-format - VBD8482

Additional information

Additional information

RealLine HPV HCR Genotype

Str, Fla