RealLine Anaplasma / Ehrlichia

The RealLine Anaplasma / Ehrlichia kit is detecting Anaplasma phagocytophilum in HEX channel and Ehrlichia chaffeensis and Ehrlichia muris in the ROX channel.

All components are lyophilized and ready-to use.



The RealLine Anaplasma / Ehrlichia kits are designed for sensitive detection of Anaplasma phagocytophilium and of Ehrlichia muris and Ehrlichia chaffensis from ticks or clinical samples by real time PCR. The kits are designed for qualitative in vitro diagnostics.

Ehrlichiosis or human granulocytic anaplasmosis are diseases which can be caused by Anaplasma phagocytophilum,Ehrlichia chaffensis or Ehrlichia muris. Humans and other mammals are infected by bites of ticks. If the disease is symptomatic it can come to flue-like symptoms up to serious symptoms like Meningoencephalitis especially in immunosupressed patients.

Sample Material

  • Blood serum, plasma
  • Cerebrospinal fluid
  • Tick suspension samples

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • HEX (535 – 554 nm) for Anaplasma phagocytophilum
  • ROX (575 – 602 nm) for Ehrlichia chaffeensis and Ehrlichia muris

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.


In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, Bio-Rad cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH)

CFX96, iQ5 iCycler (Bio-Rad, USA)

DTprime, DTLite (DNA-Technology, Russia)

Our kits are compatible to other Real-Time Cyclers. Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD5398

Additional information

Additional information

RealLine Anaplasma / Ehrlichia

Str, Fla