RealLine HBV quantitative

The RealLine HBV quantitative kit detects the different genotypes of HBV DNA by Real-time PCR. The kits provide quantitative detection.

RUO – Research Use Only

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Hepatitis B Virus HBV is a partially double-stranded DNA virus of the Hepadnaviridae family that causes acute and chronic liver disease with a very high risk of death from liver cirrhosis and cancer. Worldwide an estimated 2 billion people have been infected with the hepatitis B virus (HBV) and more than 350 million have chronic consequences of hepatitis B.

Detection channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for HBV DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.


In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH)

CFX96, iQ5 iCycler (Bio-Rad, USA)

DTprime, DTlite (DNA-Technology, Russia)

Our kits are compatible to further Real-Time cyclers. Please read the IFU properly and ask us for recommendations of device settings!

IFU - VBD0595

Additional information

Additional information

RealLine HBV quantitative RUO