RealLine Borrelia burgdorferi s.l.

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The RealLine Borrelia burgdorferi s. l. kits detect the B. burgdorferi sensu lato group (Borrelia afzelii, Borrelia garinii, Borrelia burgdorferi sensu stricto) DNA from ticks and clinical samples by Real-Time PCR.

All components are lyophilized and ready-to use.

 

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Description

Description

Lyme disease or Borreliosis is considered to be one of the fastest spreading illnesses in the world. Gram negative spirochetel bacteria from the genus Borrelia, which are collectively known as Borrelia burgdorferi sensu lato group cause Lyme disease.

Sample Material

  • Blood serum, plasma
  • Cerebrospinal fluid
  • Tick suspension samples

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for Borrelia burgdorferi s.l. DNA

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Fla-format

For Fla-format 10 Tests of the lyophilized Mastermix is filled in one vial. Pipette 300 µl of the Recovery Solution into the vial, incubate at RT for 15 min and then pipette 25 µl into your tubes or plates which fit optimally to your cycler. Add 25 µl of DNA/RNA after the extraction procedure and run the program.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

Eco48 (PCRMax, GB) for Fla-format

Our kits can be used with other Real-Time cyclers from ABI, Roche, Agilent etc.
Please read the IFU properly and ask us for recommendations of device settings!

Please log in to download the IFUs!

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Additional information

Additional information

RealLine Borrelia burgdorferi sl

Str, Fla

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