RealLine HPV 16 / 18

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The RealLine HPV 16 / 18 kit is intended for the sensitive detection and differentiation of the common high risk Genotypes 16 and 18 of the Human Papillomavirus HPV.

All components are lyophilized and ready-to use.

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Description

Description

More than 100 different Human Papilloma Viruses HPV are detected nowadays and some of these types are more likely to develop cancer in human beings. Those are  referred as “high carcinogenic risk”  or “high risk types” HPV and they are known to cause the vast majority of cervical cancers which cause death in women with an annual incidence of around half a million and a mortality of almost 50 %. But the persistent infection has also been linked to cancer of the oropharynx, larynx, vulva, vagina, penis, and anus.

Sample Material

  • Scrapings of the epithelial cells
  • Biopsies

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • HEX (535 – 554 nm) for HPV 16 DNA
  • ROX (575 – 602 nm) for HPV 18 DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH)

CFX96, iQ5 iCycler (Bio-Rad, USA)

DT96, DTLite (DNA-Technology, Russia)

Our kits are compatible with other Real-Time cyclers. Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD8473

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Additional information

Additional information

RealLine HPV 16 / 18

Str, Fla

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