RealLine Mycoplasma hominis / Mycoplasma genitalium

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The RealLine Mycoplasma hominis / Mycoplasma genitalium assay kit is intended for the detection and differentiation of Mycoplasma hominis DNA and Mycoplasma genitalium DNA by Real-time PCR.

All components are lyophilized and ready-to use.

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Description

Description

Mycoplasma genitalium is a bacterium that infects the mucous membranes of the urethra, cervix, throat and anus. Various symptoms of infection are known like urethritis (in men), discharge (both sexes), burning while urinating (both sexes), arthritis/reactive arthritis (mostly men), vaginal itching (women) as well as pain during intercourse (women).

Mycoplasma hominis is a fastidious microorganism causing systemic infections in the neonate and genital infections in the adult. It can also be the cause of serious extra-genital infections, mainly in immunosuppressed or predisposed subjects.

Sample Material

  • Saliva
  • Urine
  • Swabs of epithelial cells

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • HEX (535 – 554 nm) for Mycoplasma hominis DNA
  • ROX (575 – 602 nm) for Mycoplasma genitalium DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH)

CFX96, iQ5 iCycler (Bio-Rad, USA)

DT96, DTLite (DNA-Technology, Russia)

Our kits are compatible with other Real-Time cyclers. Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD0494

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Additional information

Additional information

RealLine Mycoplasma hominis / Mycoplasma genitalium

Str, Fla

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