RealLine Babesia species

RealLine Babesia species kit is detecting the subtypes of known Babesia species by Real-Time PCR.

All components are lyophilized and ready-to use.

RUO – Research Use Only

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Description

Description

Babesiosis is a disease caused by infection with Babesia. Babesia is a malaria-like parasite, also called a “piroplasm” that infects red blood cells. In addition to transmission by ticks, Babesia can be transmitted from mother to unborn child or through a contaminated blood transfusion. Symptoms of Babesiosis are similar to those of Lyme disease but Babesiosis more often starts with a high fever and chills. Babesiosis is often so mild it is not noticed but can be lifethreatening to people with no spleen, the elderly, and people with weak immune systems.

Sample Material

  • Blood serum, plasma
  • Cerebrospinal fluid
  • Tick suspension samples

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for Babesia species

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, Bio-Rad cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD5389

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Additional information

Additional information

RealLine Babesia species RUO

Str, Fla

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