RealLine Babesia species

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RealLine Babesia species kit is detecting the subtypes of known Babesia species by Real-Time PCR.

All components are lyophilized and ready-to use.

RUO – Research Use Only

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Description

Description

Babesiosis is a disease caused by infection with Babesia. Babesia is a malaria-like parasite, also called a “piroplasm” that infects red blood cells. In addition to transmission by ticks, Babesia can be transmitted from mother to unborn child or through a contaminated blood transfusion. Symptoms of Babesiosis are similar to those of Lyme disease but Babesiosis more often starts with a high fever and chills. Babesiosis is often so mild it is not noticed but can be lifethreatening to people with no spleen, the elderly, and people with weak immune systems.

Sample Material

  • Blood serum, plasma
  • Cerebrospinal fluid
  • Tick suspension samples

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for Babesia species

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Fla-format

For Fla-format 10 Tests of the lyophilized Mastermix is filled in one vial. Pipette 300 µl of the Recovery Solution into the vial, incubate at RT for 15 min and then pipette 25 µl into your tubes or plates which fit optimally to your cycler. Add 25 µl of DNA/RNA after the extraction procedure and run the program.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

Eco48 (PCRMax, GB) for Fla-format

Our kits can be used with other Real-Time cyclers from ABI, Roche, Agilent etc.
Please read the IFU properly and ask us for recommendations of device settings!

Please log in to download the IFUs!

Additional information

Additional information

RealLine Babesia species RUO

Str, Fla

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