RealLine HCV quantitative

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The RealLine HCV quantitative Kit is intended for the sensitive detection of Hepatitis-C Virus and the determination of the viral load by quantification.

RUO – Research Use Only

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Description

Description

Hepatitis C Virus (HCV) is a public health concern worldwide and a major cause of chronic liver inflammation, cirrhosis and hepatocellular carcinoma. The Hepatitis C virus is transmitted parenterally, showing a high incidence in hemophiliacs hemodialysis patients, injecting drug users and patients with post-transfusion hepatitis.

Detection channels

  • FAM (495 – 520 nm) for Internal Control
  • ROX (575 – 602 nm) for HCV cDNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, Bio-Rad cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH)

CFX96, iQ5 iCycler (Bio-Rad, USA)

DTprime, DTlite (DNA-Technology, Russia)

Our kits are compatible to further Real-Time cyclers. Please read the IFU properly and ask us for recommendations of device settings!

IFU - VBD0794

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Additional information

Additional information

RealLine HCV quantitative RUO

Str

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