RealLine VZV

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The RealLine VZV kit is designed for sensitive detection of Varicella- Zoster-Virus DNA in clinical samples by Real-Time PCR.

All components are lyophilized and ready-to use.

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Description

Description

Primary Varicella-Zoster-Virus VZV infection causes chicken-pox in children and serious complications of shingles include postherpetic neuralgia, zoster multiplex, myelitis, herpes ophthalmicus, or zoster sine herpete in adults and rarely in children. In about 10 – 20 % of the cases, VZV reactivates later in life. It more commonly occurs in people over age 50, and in those who have a weakened immune system.

Sample Material

  • Epithelial cell swabs
  • Tissue fluid from erosive-ulcerative skin lesions and mucosa
  • Urine, saliva,
  • Blood serum and plasma.

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for Varicella-Zoster-Virus VZV DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Fla-format

For Fla-format 10 Tests of the lyophilized Mastermix is filled in one vial. Pipette 300 µl of the Recovery Solution into the vial, incubate at RT for 15 min and then pipette 25 µl into your tubes or plates which fit optimally to your cycler. Add 25 µl of DNA/RNA after the extraction procedure and run the program.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

Eco48 (PCRMax, GB) for Fla-format

Our kits can be used with other Real-Time cyclers from ABI, Roche, Agilent etc.
Please read the IFU properly and ask us for recommendations of device settings!

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Additional information

Additional information

RealLine VZV

Str, Fla

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