RealLine VZV

The RealLine VZV kit is designed for sensitive detection of Varicella- Zoster-Virus DNA in clinical samples by Real-Time PCR.

All components are lyophilized and ready-to use.

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Description

Description

Primary Varicella-Zoster-Virus VZV infection causes chicken-pox in children and serious complications of shingles include postherpetic neuralgia, zoster multiplex, myelitis, herpes ophthalmicus, or zoster sine herpete in adults and rarely in children. In about 10 – 20 % of the cases, VZV reactivates later in life. It more commonly occurs in people over age 50, and in those who have a weakened immune system.

Sample Material

  • Epithelial cell swabs
  • Tissue fluid from erosive-ulcerative skin lesions and mucosa
  • Urine, saliva,
  • Blood serum and plasma.

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for Varicella-Zoster-Virus VZV DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, Bio-Rad cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD2185

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Additional information

Additional information

RealLine VZV

Str, Fla

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