RealLine Ureaplasma urealyticum

The RealLine Ureaplasma urealyticum kit detects Ureaplasma urealyticum DNA in clinical samples by Real-Time PCR. The kits are designed for in vitro diagnostics and qualitative detection.

All components are lyophilized and ready-to use.

 

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Description

Description

Ureaplasma urealyticum is part of the normal genital flora of both men and women. It is found in about 70 % of sexually active humans. Ureaplasma urealyticum had also been described to be associated with a number of diseases in humans, including non-specific urethritis, infertility, chorioamnionitis, stillbirth, premature birth and in the perinatal period, pneumonia, bronchopulmonary dystroplasia as well as meningitis.

Sample Material

  • Scrapings of epithelial cells
  • Urine
  • Semen
  • Prostatic juice

Detection channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for Ureaplasma urealyticum DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, Bio-Rad cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD2298

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Additional information

Additional information

RealLine Ureaplasma urealyticum

Str, Fla

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