RealLine Trichomonas vaginalis / Neisseria gonorrhoeae

The RealLine Trichomonas vaginalis / Neisseria gonorrhoeae kit is intended for a detection and differentiation of  Trichomonas vaginalis and Neisseria gonorrhoeae DNA in clinical samples by Real-Time PCR.

All components are lyophilized and ready-to use.

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Description

Description

Trichomonas vaginalis – a flagellate – causes trichomoniasis, a disease that causes vaginal infections in women and urethritis in men. The transmission of trichomoniasis runs through sexual intercourse and is one of the most common sexually transmitted diseases.
Neisseria gonorrhoeae are gram-negative motile bacteria and one of the most common sexually transferred diseases. In women N. gonorrhoeae can lead to severe symptoms such as infertility and abortion. In men N. gonorrhoeae can lead to painful urethritis.

Sample Material

  • Epithelial cells swabs
  • Semen
  • Prostatic fluid
  • Urine

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • HEX (535 – 554 nm) for Neisseria gonorrhoeae DNA
  • ROX (575 – 602 nm) for Trichomonas vaginalis DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, Bio-Rad cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD0496

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Additional information

Additional information

RealLine Trichomonas vaginalis / Neisseria gonorrhoeae

Str, Fla

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