RealLine SARS-CoV-2 lyo

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The RealLine SARS-CoV-2 lyo kit is intended for the detection of RNA of RdRP-Gene and N-Gene from SARS-CoV-2 by Real-Time-PCR method.

All components are lyophilized and ready-to use.



The RealLine SARS-CoV-2 lyo kit detects the Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2, formerly 2019-nCoV). This novel virus belongs to the corona virus family and from January 2020, it was discovered that this virus is responsible for infections that spread rapidly around the world. A few days after the first report of patients with pneumonia of unclear origin, SARS-CoV-2 was identified as the causative agent. SARS-CoV-2 is mainly transmitted by droplet infection when coughing or sneezing and in close contact with infected persons.

Sample Material

  • Nasopharyngeal and oropharyngeal swabs
  • Sputum,
  • Bronchoalveolar lavage
  • Fluid

Detection Channels

  • FAM for Internal Control (RNA-IC)
  • ROX for RdRP- and N-genetargets of the SARS-CoV-2 Virus RNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.


In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Please read the IFU properly and ask us for recommendations of device settings!

Please log in to download the IFUs!

Additional information

Additional information

RealLine SARS-CoV-2 lyo RUO