RealLine Rubella

The RealLine Rubella kit is designed for sensitive detection of Rubella RNA in clinical samples by one-step Reverse Transcription and Real-Time PCR.

All components are lyophilized and ready-to use.

 

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Description

Description

Rubella virus is a single-stranded RNA virus and the contagious infection with Rubella occurs worldwide. The virus is extremely dangerous for pregnant women that have no rubella immunity, since Rubella can infect the fetus causing Congenital Rubella Syndrome (CRS). In the first trimester of pregnancy, CRS is more likely to cause major defects and often leads to miscarriage or a stillborn baby. After the 20th week of pregnancy, the problems are rare.

Sample Material

  • Blood serum, plasma

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for Rubella RNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format:

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, Bio-Rad cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD2598-R

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Additional information

Additional information

RealLine Rubella

Str, Fla

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