RealLine Mycobacterium tuberculosis

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The RealLine Mycobacterium tuberculosis kit is designed to detect Mycobacterium tuberculosis complex containing mycobacteria causing tuberculosis in humans: M. tuberculosis, M. bovis, M. bovis BCG, M. microti, M. africanum

Target Genes: IS6110 AND IS1081

All components are lyophilized and ready-to use

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Description

Description

Tuberculosis, also named MBT, TBC or TB, is a world-wide spread, common and often lethal infectious disease. The number of new infections is still increasing. Tuberculosis usually attacks the lungs, but also other parts of the body can be affected. The infection is spread by droplet infection and causes symptoms, like chronic cough with blood-tinged sputum, fever, night sweats and weight loss.

Sample Material

  • Whole blood
  • Blood serum (plasma)
  • Biopsy material
  • Urine
  • Bronchopulmonary lavage
  • Sputum
  • Synovial fluid
  • Cerebrospinal fluid

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for Mycobacterium tuberculosis complex DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format (96 Tests without extraction components):

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Uni-format (48 tests with extraction):

The Ready-Mastermix is lyophilized  and prepared in 0.2 ml clear single tubes.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes their caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, Rotor-Gene Cyclers. BIO-RAD cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni format

Our kits can be used with other Real-Time cyclers from ABI, Roche, Agilent etc.
Please read the IFU properly and ask us for recommendations of device settings!

Please log in to download the IFUs!

Additional information

Additional information

RealLine Mycobacterium tuberculosis incl. extraction

Uni, Str

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