RealLine HHV – 6

The RealLine HHV-6 kits are designed for sensitive detection of HHV-6 DNA in clinical samples by Real-Time PCR.

All components are lyophilized and ready-to use.

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HHV-6 infections can asymptomatically occur early in life with lifelong persistence. Primary infection with HHV-6 causes roseola infantum, a common childhood disease that resolves spontaneously. The virus remains latent in lymphocytes and monocytes and persists at low levels in cells and tissues. It can reactivate at a later date, and is believed to contribute to diseases of the bone marrow and/or central nervous system in some patients, including mononucleosis like illness with fever, lymphadenopathy, hepatitis, fatal encephalitis, chronic fatigue syndrome, and possibly multiple sclerosis. Transmission of HHV-6 is believed to occur as a result of exposure to saliva.


Clinical Material

  • Blood samples (serum, plasma)
  • Scrapings of epithelial cells
  • Saliva
  • Semen
  • Prostatic fluid
  • Urine
  • Whole blood,
  • Leukocytic mass

Detection channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for HHV-6 DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.


In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, Bio-Rad cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Please read the IFU properly and ask us for recommendations of device settings!

IFU - VBD2150

Additional information

Additional information

RealLine HHV - 6

Str, Fla