RealLine HCV quantitative, incl. Extraction

The RealLine HCV quantitative Kit is intended for the sensitive detection of Hepatitis-C Virus.

The components for the extraction of the RNA are included.

RUO – Research Use Only

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Hepatitis C virus (HCV) is a public health concern worldwide and a major cause of chronic liver inflammation, cirrhosis and hepatocellular carcinoma. The Hepatitis C Virus is transmitted parenterally, showing a high incidence in hemophiliacs hemodialysis patients, injecting drug users and patients with post-transfusion hepatitis.

Detection channels

  • FAM (495 – 520 nm) for Internal Control
  • ROX (575 – 602 nm) for HCV cDNA


The Ready-Mastermix is lyophilized  and prepared in 0.2 ml clear single tubes.  50 µl of extracted RNA have to be pipetted to the tubes, close the tubes their caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, Rotor-Gene Cyclers. Bio-Rad cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

If you have questions to the use of your cycler, please contact us!

IFU - VBD0799

Additional information

Additional information

RealLine HCV quantitative, incl. extraction RUO