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RealLine HCV Genotype quantitative

RealLine HCV Genotype quantitative Kits are intended for the sensitive detection, quantification and differentation of HCV genotypes 1/2/3

RUO – Research Use Only

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Description

Description

Description

Hepatitis C Virus (HCV) is a public health concern worldwide and a major cause of chronic liver inflammation, cirrhosis and hepatocellular carcinoma. The Hepatitis C Virus is transmitted parenterally, showing a high incidence in hemophiliacs hemodialysis patients, injecting drug users and patients with post-transfusion hepatitis.

Detection channels

  • FAM (495 – 520 nm) for HCV-2 cDNA (Tube 1) and Internal Control (Tube 2)
  • HEX (535 – 554 nm) for HCV-1 cDNA (Tube 1)
  • ROX (575 – 602 nm) for HCV-3 cDNA (Tube 1) and all HCV cDNA (Tube 2)

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Format

Str-format:

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Validated Cyclers

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

If you have questions to the use of your cycler, please contact us!

Datasheets

IFU - VBD0797

pdf
Additional information

Additional information

RealLine HCV Genotype quantitative RUO

Str
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