RealLine HBV / HCV / HIV

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The RealLine HBV / HCV / HIV kit is intended for the differential detection of HCV, HBV, HIV Type 1 and HIV Type 2 by Real-time PCR in one test.

RUO – Research Use Only

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Description

Description

The assay kit is intended for the qualitative and differential detection of DNA of Hepatitis B-Virus (HBV) and RNA of the Hepatitis C-Virus (HCV) and the Human immunodefiency Virus (HIV) Types 1 and Type 2.

Sample Material

Blood serum and plasma

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • HEX (535 – 554 nm) for HCV RNA (Mastermix 1) and HIV-2 cDNA (Mastermix 2)
  • ROX (575 – 602 nm) for HBV DNA (Mastermix 1) and HIV-1 cDNA (Mastermix 2)

Str-format:

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Please get in contact with us, if you have questions to other cyclers

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Additional information

Additional information

RealLine HBV / HCV / HIV - RUO

Str

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