RealLine HBV / HCV / HIV

The RealLine HBV / HCV / HIV kit is intended for the differential detection of HCV, HBV, HIV Type 1 and HIV Type 2 by Real-time PCR in one test.

RUO – Research Use Only



The assay kit is intended for the qualitative and differential detection of DNA of Hepatitis B-Virus (HBV) and RNA of the Hepatitis C-Virus (HCV) and the Human immunodefiency Virus (HIV) Types 1 and Type 2.

Sample Material

Blood serum and plasma

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • HEX (535 – 554 nm) for HCV RNA (Mastermix 1) and HIV-2 cDNA (Mastermix 2)
  • ROX (575 – 602 nm) for HBV DNA (Mastermix 1) and HIV-1 cDNA (Mastermix 2)


In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, Bio-Rad cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH)

CFX96, iQ5 iCycler (Bio-Rad, USA)

DT96, DTLite (DNA-Technology, Russia)

Our kits are compatible to further Real-Time cyclers. Please read the IFU properly and ask us for recommendations of device settings!

IFU - VBD0592


For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Additional information

Additional information

RealLine HBV / HCV / HIV - RUO