RealLine Helicobacter pylori

The RealLine Helicobacter pylori kits detects Helicobacter pylori DNA in clinical samples by Real-Time PCR. The kits are designed for in vitro diagnostics and provide qualitative detection.

All components are lyophilized and ready-to use

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Description

Description

Helicobacter pylori infection is usually acquired in early childhood in all countries. Over 80 % of individuals infected with the bacterium are asymptomatic. H. pylori is contagious, person-to-person transmission by oral-oral or fecal-oral route is most likely. It can also be spread through contaminated food or water.
The bacterium causes a chronic low-level inflammation of the stomach lining and is strongly linked to the development of chronic gastritis, duodenal and gastric ulcers, gastric mucosa-associated lymphoid tissue lymphoma and gastric adenocarcinoma.

Sample Material

  • Stomach tissue samples

Detection Channels

  • FAM (495 – 520 nm) for Helicobacter Internal Control (IC)
  • ROX (575 – 602 nm) for Helicobacter pylori DNA

Str-format:

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD3798

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Additional information

Additional information

RealLine Helicobacter pylori

Str, Fla

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