RealLine Helicobacter pylori

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The RealLine Helicobacter pylori kits detects Helicobacter pylori DNA in clinical samples by Real-Time PCR. The kits are designed for in vitro diagnostics and provide qualitative detection.

All components are lyophilized and ready-to use

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Description

Description

Helicobacter pylori infection is usually acquired in early childhood in all countries. Over 80 % of individuals infected with the bacterium are asymptomatic. H. pylori is contagious, person-to-person transmission by oral-oral or fecal-oral route is most likely. It can also be spread through contaminated food or water.
The bacterium causes a chronic low-level inflammation of the stomach lining and is strongly linked to the development of chronic gastritis, duodenal and gastric ulcers, gastric mucosa-associated lymphoid tissue lymphoma and gastric adenocarcinoma.

Sample Material

  • Stomach tissue samples

Detection Channels

  • FAM (495 – 520 nm) for Helicobacter Internal Control (IC)
  • ROX (575 – 602 nm) for Helicobacter pylori DNA

Str-format:

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Fla-format:

For Fla-format 10 Tests of the lyophilized Mastermix is filled in one vial. Pipette 300 µl of the Recovery Solution into the vial, incubate at RT for 15 min and then pipette 25 µl into your tubes or plates which fit optimally to your cycler. Add 25 µl of DNA/RNA after the extraction procedure and run the program.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

Eco48 (PCRMax, GB) for Fla-format

Our kits can be used with other Real-Time cyclers from ABI, Roche, Agilent etc.
Please read the IFU properly and ask us for recommendations of device settings!

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Additional information

Additional information

RealLine Helicobacter pylori

Str, Fla

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