The RealLine Gardnerella vaginalis kit detects the Gardnerella vaginalis DNA in clinical samples by real-time PCR.
All components are lyophilized and ready-to use.
Gardnerella vaginalis is a facultatively Gram-variable rod that is involved, together with many other bacteria, mostly anaerobic, in bacterial vaginosis in some women as a result of a disruption in the normal vaginal microflora.
Sample Material
Detection Channels
For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.
Str-format
In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted. 50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.
Fla-format
For Fla-format 10 Tests of the lyophilized Mastermix is filled in one vial. Pipette 300 µl of the Recovery Solution into the vial, incubate at RT for 15 min and then pipette 25 µl into your tubes or plates which fit optimally to your cycler. Add 25 µl of DNA/RNA after the extraction procedure and run the program.
RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format
CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format
DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format
Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format
Eco48 (PCRMax, GB) for Fla-format
Our kits can be used with other Real-Time cyclers from ABI, Roche, Agilent etc.
Please read the IFU properly and ask us for recommendations of device settings!
| RealLine Gardnerella vaginalis | Str, Fla |
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