RealLine EBV quantitative

The RealLine EBV quantitative kits are designed for sensitive detection of Eppstein-Barr-Virus DNA in clinical samples by Real-Time PCR. The kits are designed for in vitro diagnostics and can be used for a quantitative determination of the viral load.

All components are lyophilized and ready-to use.


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The Eppstein-Barr-Virus EBV has infected more than 90 % of the world’s population, leaving the majority of individuals with a lifelong silent infection. Many children become infected with EBV asymptomatically. But the virus can also cause severe diseases and is the causative agent of infectious mononucleosis and others. EBV is associated with the development of two epithelial tumors, nasopharyngeal carcinoma and oral hairy leukoplakia.

Sample Material

  • Blood serum, plasma
  • Cerebrospinal fluid
  • Saliva
  • Biopsy materials
  • Orophyngeal swabs
  • Bronchopulmonary lavage
  • Whole blood

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for Eppstein-Barr Virus EBV DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.


In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.


For Fla-format 10 Tests of the lyophilized Mastermix is filled in one vial. Pipette 300 µl of the Recovery Solution into the vial, incubate at RT for 15 min and then pipette 25 µl into your tubes or plates which fit optimally to your cycler. Add 25 µl of DNA/RNA after the extraction procedure and run the program.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

Eco48 (PCRMax, GB) for Fla-format

Our kits can be used with other Real-Time cyclers from ABI, Roche, Agilent etc.
Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD2196


IFU Str-format - VBD2198

Additional information

Additional information

RealLine EBV quantitative

Str, Fla