RealLine Chlamydia trachomatis / Ureaplasma urealyticum

The RealLine Chlamydia trachomatis / Ureaplasma urealyticum kits are designed for a differential and sensitive detection of  the plasmid and genomic DNA of Chlamydia trachomatis and the DNA of Ureaplasma urealyticum in clinical samples by Real- Time PCR. The kits are for qualitative diagnosis.

All components are lyophilized and ready-to use.

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Untreated infections by Chlamydia trachomatis can cause serious reproductive and other health problems with both short-term and long-term consequences. In both sexes Chlamydia infection can cause proctitis, trachoma and infertility as well as prostatis, epidiymitis in men and cervictis, PID, ectopic pregnancy and acute/chronic pelvic pain in women.

Ureaplasma urealyticum is commonly found in the urogenital tracks of human beings, but overgrowth can lead to infections that cause the patient discomfort, and especially infection with U. urealyticum in pregnancy and birth can be complicated.

Sample Material

  • Scrapings of epithelial cells (urethral, cervical, vaginal)
  • Semen
  • Prostatic fluid
  • Urine

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • HEX (535 – 554 nm) for Chlamydia trachomatis DNA
  • ROX (575 – 602 nm) for Ureaplasma urealyticum DNA


For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.


In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, Bio-Rad cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH)

CFX96, iQ5 iCycler (Bio-Rad, USA)

DTprime, DTLite (DNA-Technology, Russia)

Our kits are compatible to other Real-Time Cyclers. Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD0492

Additional information

Additional information

RealLine Chlamydia trachomatis / Ureaplasma urealyticum

Str, Fla