RealLine Chlamydia trachomatis / Mycoplasma genitalium

The RealLine Chlamydia trachomatis / Mycoplasma genitalium kits are designed for a differential and sensitive detection of the plasmid and genomic DNA of Chlamydia trachomatis and the DNA of Mycoplasma genitalium in clinical samples by Real- Time PCR. The kits are designed for qualitative diagnosis.

All components are lyophilized and ready-to use.

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Untreated infections by Chlamydia trachomatis can cause serious reproductive and other health problems with both short-term and long-term consequences. In both sexes Chlamydia infection can cause proctitis, trachoma and infertility as well as prostatis, epidiymitis in men and cervictis, PID, ectopic pregnancy and acute/chronic pelvic pain in women.

Mycoplasma genitalium is a bacterium that infects the mucous membranes of the urethra, cervix, throat and anus. Various symptoms of infection are known like urethritis (in men), discharge (both sexes), burning while urinating (both sexes), arthritis/reactive arthritis (mostly men), vaginal itching (women) as well as pain during intercourse (women).

Sample Material

  • Scrapings of epithelial cells (urethral, cervical, vaginal)
  • Semen
  • Prostatic fluid
  • Urine

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • HEX (535 – 554 nm) for Chlamydia trachomatis DNA
  • ROX (575 – 602 nm) for Mycoplasma genitalium DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.


In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, Bio-Rad cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD0490

Additional information

Additional information

RealLine Chlamydia trachomatis / Mycoplasma genitalium

Str, Fla