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RealLine Chlamydophila pneumoniae

RealLine Chlamydophila pneumoniae Kits detect Chlamydophila pneumoniae DNA in clinical samples by Real-Time PCR.

All components are lyophilized and ready-to use

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Description

Description

Description

Chlamydophila pneumoniae, an obligate intracellular pathogen, is a causal agent of community-acquired pneumonia (CAP) and is categorized as an atypical cause of pneumonia. The clinical symptoms of C. pneumoniae infection are nonspecific and do not differ significantly from those caused by other atypical organisms, such as Mycoplasma pneumoniae and respiratory viruses

Sample Material

  • Nasopharyngeal swabs
  • Oropharyngeal swabs
  • Nasopharyngeal aspirate
  • Sputum
  • Bronchoalveolar lavage fluid
  • Bronchial washings

Detection channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • ROX (575 – 602 nm) for Chlamydophila pneumoniae DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Formats

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are medium-profile tubes and fit to RealLine Cyclers, BIO-RAD cyclers and others.

Fla-format

For Fla-format 10 Tests of the lyophilized Mastermix is filled in one vial. Pipette 300 µl of the Recovery Solution into the vial, incubate at RT for 15 min and then pipette 25 µl into your tubes or plates which fit optimally to your cycler. Add 25 µl of DNA/RNA after the extraction procedure and run the program.

Validated Cyclers

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany) for Uni and Fla format

Eco48 (PCRMax, GB) for Fla-format

Our kits can be used with other Real-Time cyclers from ABI, Roche, Agilent etc.
Please read the IFU properly and ask us for recommendations of device settings!

Datasheets

IFU Str-Format - VBD5594

pdf

IFU Fla-format -VBD5595

pdf
Additional information

Additional information

RealLine Chlamydia trachomatis

Str, Fla
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