RealLine Candida albicans / Gardnerella vaginalis

The RealLine Candida albicans / Gardnerella vaginalis kit is detecting and differentiating DNA from Candida albicans and Gardnerella vaginalis by Real-Time PCR.

All components are lyophilized and ready-to use.

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Description

Description

Candida albicans can cause Candidiasis. Such infections range from superficial, such as oral thrush and vaginitis, to systemic and potentially life-threatening diseases.  Candida infections of the latter category are also referred to as candidemia and are usually confined to severely immunocompromised persons, such as cancer, transplant, and AIDS patients, as well as nontrauma emergency surgery patients.

Gardnerella vaginalis is a facultatively anaerobic Gram-variable rod that can cause bacterial vaginosis in some women as a result of a disruption in the normal vaginal microflora.

Sample Material

  • Saliva
  • Urine
  • Scrapings of epithelial cells

Detection Channels

  • FAM (495 – 520 nm) for Internal Control (IC)
  • HEX (535 – 554 nm) for Candida albicans DNA
  • ROX (575 – 602 nm) for Gardnerella vaginalis DNA

For optimal results please always use the RealLine Internal Control. In case you do not use RealLine Extraction kits, order RealLine Internal Control (REF VBC8881) seperately.

Str-format

In Str-format kits the Ready-Mastermix is lyophilized and prepared in 0.2 ml white tubes arranged in a plate. For the preparation the respective number of tubes can be easily cutted.  50 µl of extracted DNA/RNA have to be pipetted to the tubes, close the tubes with PCR- foil or caps and run the test. The tubes are regular-profile tubes and fit to RealLine Cyclers, Bio-Rad cyclers and others.

RealLine Cycler 48 or RealLine Cycler 96 (BIORON Diagnostics GmbH) for Str, Fla and Uni format

CFX96, iQ5 iCycler (Bio-Rad, USA) for Str, Fla and Uni format

DT96, DTLite (DNA-Technology, Russia) for Str, Fla and Uni format

Please read the IFU properly and ask us for recommendations of device settings!

IFU Str-format - VBD0444

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Additional information

Additional information

RealLine Candida albicans / Gardnerella vaginalis

Str, Fla

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