Treponema pallidum

In vitro Diagnostics


Product name Cat.No. Format Packsize
RealLine Treponema pallidum (Str-format)
VBD1898 Str 48 Tests
RealLine Treponema pallidum (Fla-format)
VBD1896 Fla 50 Tests

Treponema pallidum is a motile spirochaete that is generally acquired by close sexual contact, entering the host via breaches in squamous or columnar epithelium. T. pallidum is causing Syphilis is a sexually transmitted infection.The primary route of transmission is through sexual contact; it may also be transmitted from mother to fetus during pregnancy or at birth, resulting in congenital syphilis.

Typical for this disease is a beginning with painless mucosal ulcers and swollen lymph nodes. In some of the infected there is a chronic course, which is characterized by a variety of skin and organ involvement. In the final stage it comes to the destruction of the central nervous system.


RealLine Treponema pallidum (Fla-format) kit is designed for the analysis of clinical specimen (scrapings of epithelial cells and tissue fluid from erosive and ulcerative lesions of the skin and mucous membranes)

The assay is based on the real-time polymerase chain reaction (PCR) method with fluorescent detection of the amplified product.

Sample Material

  • Scrapings of cells (see decription in IFU)

Real-Time PCR Detection Channels

  • FAM (495-520 nm)
  • Internal Control IC
  • ROX (575-602 nm)
  • Treponema pallidum and Positive Control

Validated Cyclers

  • RealLine Cycler 96 and RealLine Cycler 48 (BIORON Diagnostics GmbH)
  • Str, Fla, Uni
  • iQ™ iCycler, iQ5™ iCycler, CFX96™ (Bio-Rad, USA)
  • Str, Fla, Uni
  • DT96, DTLite (DNA-Technology, Russia)
  • Str, Fla, Uni
  • Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany)
  • Uni, Fla
  • Eco48, (PCRMax, GB)
  • Fla

Our kits can be used with other real-time cyclers from companies ABI, ROCHE, Agilent etc. Please read the IFU properly, and ask us for recommendations for the settings.