Rickettsia species

RUO

Product name Cat.No. Format Packsize
RealLine Rickettsia species (Str-format), RUO
[PDS]
VBD5391 Str 48 Tests
RealLine Rickettsia species (Fla-format), RUO
[PDS]
VBD5392 Fla 50 Tests

Applications

Rickettsia are world-wide organisms, which are found in many ticks, fleas, mites and lice and serve as vectors. Endemic in Mediterranean countries, Eastern Europe, the tropics and North America In humans, they cause a whole range of diseases with different clinical pictures, which are grouped together medically to the group of Rickettsioses. Examples include typhus (syn. Typhus exanthematicus), rickettsialpox, Brill-Zinsser disease, Boutonneuse fever (Mediterranean tick-borne fever) and Rocky Mountain spotted fever. Like viruses, Rickettsiae thrive as intracellular parasites exclusively in living cells. In this way, they manage to escape the immune system of their hosts.

Sample material

  • Blood serum, plasma
  • Leukocyte blood fraction
  • Biopsy materials
  • Whole blood
  • Inoculation eschar swabs
  • Tick suspensions

Real-Time PCR Detection Channels

  • FAM (495-520 nm)
  • Internal Control IC
  • ROX (575-602 nm)
  • Rickettsia DNA and Positive Control

Validated Cyclers

  • RealLine Cycler 96 and RealLine Cycler 48 (BIORON Diagnostics GmbH)
  • Str, Fla, Uni
  • iQ™ iCycler, iQ5™ iCycler, CFX96™ (Bio-Rad, USA)
  • Str, Fla, Uni
  • DT96, DTLite (DNA-Technology, Russia)
  • Str, Fla, Uni
  • Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany)
  • Uni, Fla

Our kits can be used with other real-time cyclers from companies ABI, ROCHE, Agilent etc. Please read the IFU properly, and ask us for recommendations for the settings.