RealLine Human Papilloma Viruses HPV

HPV High Carcinogenic Risk Genotype

In vitro diagnosticum


Product name Cat.No. Format Packsize
RealLine HPV HCR Genotype Quantitative (Str-format), CE - IVD
VBD8478 Str 96 Tests
RealLine HPV HCR Genotype (Str-format), CE - IVD
VBD8479 Str 96 Tests
RealLine HPV HCR Genotype (Fla-format), CE - IVD
VBD8482 Fla 99 Tests

HPV types that are more likely to lead to the development of cancer are referred as “high carcinogic risk” types HPV. High-risk HPV-types are known to cause the vast majority of cervical cancers which cause death in women with an annual incidence of around half a million and a mortality of almost 50 %.

These kits are detecting and differentiating HPV high carcinogenic risk genotypes:

16, 18, 21, 33, 35, 39, 45, 51, 52, 56, 58, and 59.


RealLine HPV High Risk Genotype kit is designed for the analysis of clinical materials:
scrapings of the epithelial cells, semen, prostatic secretions, and urine.
The assay is based on the real-time polymerase chain reaction (PCR) method with fluorescent
detection of the amplified product.

Sample Material

  • scrapings of the epithelial cells
  • semen
  • prostatic secretions
  • urine

Real-Time PCR Detection Channels

  • FAM (495-520 nm)
  • HPV genotypes
  • HEX (535-554 nm)
  • HPV genotypes
  • ROX (575-602 nm)
  • HPV genotypes
  • Cy5 (647-667 nm)
  • Internal Control IC and ß-Actin

Validated Cyclers

  • RealLine Cycler 96 and RealLine Cycler 48 (BIORON Diagnostics GmbH)
  • Str, Fla, Uni
  • iQ™ iCycler, iQ5™ iCycler, CFX96™ (Bio-Rad, USA)
  • Str, Fla, Uni
  • DT96, DTLite (DNA-Technology, Russia)
  • Str, Fla, Uni
  • Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany)
  • Uni, Fla
  • Eco48, (PCRMax, GB)
  • Fla

Our kits can be used with other real-time cyclers from companies ABI, ROCHE, Agilent etc. Please read the IFU properly, and ask us for recommendations for the settings.