RealLine KRAS Detect


Product name Cat.No. Packsize
RealLine KRAS Detect, Kit for Real Time PCR
MED20401 12 Tests
RealLine KRAS Detect, Kit for Real Time PCR
MED20410 36 Tests

Colorectal cancer (mCRC) is the fourth most common cancer among men and women. About 40% of mCRC patients have mutations of the KRAS gene in the tumor. Tumors with KRAS mutations in codons 12 or 13 are resistant to anti-EGFR therapy. Accordingly, anti-EGFR therapy of mCRC using Erbitux® or Vectibix® is recommended only for tumors without such mutations.

Mutant codon – Nucleotides

  • G12C – TGT
  • G12S – AGT
  • G12R – CGT
  • G12V – GTT
  • G12D – GAT
  • G12A – GCT
  • G13D – GAC


The RealLine KRAS Detect kit is designed for the detection of 7 mutations in the codons 12 and 13 of the kRas gene by real-time PCR.

Indications for testing

Targeted therapy of metastatic colorectal cancer (mCRC).

Sample Material: DNA extracted from

  • fresh tissue
  • fresh-frozen tissue
  • formalin fixed paraffin embedded (FFPE) tissue

Real-Time PCR Detection Channels

  • FAM (495-520 nm)
  • KRAS mutations and Positive Control
  • ROX (575-602 nm)
  • Internal Control IC

Validated Cyclers

  • RealLine Cycler 96 and RealLine Cycler 48 (BIORON Diagnostics GmbH)
  • Str, Fla, Uni
  • iQ™ iCycler, iQ5™ iCycler, CFX96™ (Bio-Rad, USA)
  • Str, Fla, Uni
  • DT96, DTLite (DNA-Technology, Russia)
  • Str, Fla, Uni

Our kits can be used with other real-time cyclers from companies ABI, ROCHE, Agilent, Qiagen etc. Please read the IFU properly, and ask us for recommendations for the settings.