RealLine Anaplasma / Ehrlichia

In vitro diagnosticum

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Product name Cat.No. Format Packsize
RealLine Anaplasma / Ehrlichia (Str-format), CE - IVD
[PDS]
VBD5398 Str 48 Tests
RealLine Anaplasma / Ehrlichia (Fla-format), CE - IVD
[PDS]
VBD5399 Fla 50 Tests

This kit is detecting Anaplasma phagocytophilum in one channel and Ehrlichia chaffeensis and E. muris in the second channel.

Ehrlichiosis or human granulocytic anaplasmosis are diseases which can be caused by Anaplasma phagocytophilum, Ehrlichia chaffensis or Ehrlichia muris. Humans and other mammals are infected by bites of ticks. If the disease is symptomatic it can come to flue-like symptoms up to serious symptoms like Meningoencephalitis especially in immunosupressed patients.

Applications

The RealLine Anaplasma / Ehrlichia kits are designed for sensitive direct detection of Anaplasma phagocytophilium and of Ehrlichia muris and E. chaffensis in clinical samples by real time PCR. The kits are designed for qualitative in vitro diagnostics.

Sample Material

  • Blood serum, plasma
  • Cerebrospinal fluid
  • Tick suspension samples

Real-Time PCR Detection Channels

  • FAM (495-520 nm)
  • Internal Control IC
  • HEX (535-554 nm)
  • Anaplasma phagocytophilium
  • ROX (575-602 nm)
  • Ehrlichia muris and E. chaffeensis

Validated Cyclers

  • RealLine Cycler 96 and RealLine Cycler 48 (BIORON Diagnostics GmbH)
  • Str, Fla, Uni
  • iQ™ iCycler, iQ5™ iCycler, CFX96™ (Bio-Rad, USA)
  • Str, Fla, Uni
  • DT96, DTLite (DNA-Technology, Russia)
  • Str, Fla, Uni
  • Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany)
  • Uni, Fla
  • Eco48, (PCRMax, GB)
  • Fla

Our kits can be used with other real-time cyclers from companies ABI, ROCHE, Agilent etc. Please read the IFU properly, and ask us for recommendations for the settings.