RealLine Human Papilloma Viruses HPV

HPV HCR Screen

In vitro diagnosticum

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Product name Cat.No. Format Packsize
RealLine HPV HCR Screen (Str-format), CE-IVD
[PDS]
VBD8444 Str 96 Tests
Screening Set: High and Low Carcinogenic Risk Types (Str-format)
VBD8444Set Str 1 Set
RealLine HPV HCR Screen (Fla-format), CE - IVD
[PDS]
VBD8445 Fla 100 Tests
Screening Set: High and Low Carcinogenic Risk Types (Fla-format), CE - IVD
VBD8445Set Fla 1 Set

Applications

RealLine HPV High Risk Genotype kit is designed for the analysis of clinical materials:
scrapings of the epithelial cells, semen, prostatic secretions, and urine.

The assay is based on the real-time polymerase chain reaction (PCR) method with fluorescent detection of the amplified product.

HPV types that are more likely to lead to the development of cancer are referred as “high carcinogic risk” types HPV. High-risk HPV-types are known to cause the vast majority of cervical cancers which cause death in women with an annual incidence of around half a million and a mortality of almost 50 %.

These kits are detecting HPV high carcinogenic risk genotypes:

16, 18, 31, 33, 35, 39, 45, 51, 52, 56, 58, 59, 66 and 68.

Sample Material

  • scrapings of the epithelial cells
  • semen
  • prostatic secretions
  • urine

Real-Time PCR Detection Channels

Mastermix 1:

  • FAM (495-520 nm)
  • Internal Control IC
  • HEX (535-554 nm)
  • HPV genotypes
  • ROX (575-602 nm)
  • HPV genotypes

Mastermix 2:

  • FAM (495-520 nm)
  • Internal Control IC
  • ROX (575-602 nm)
  • HPV genotypes

Validated Cyclers

  • RealLine Cycler 96 and RealLine Cycler 48 (BIORON Diagnostics GmbH)
  • Str, Fla, Uni
  • iQ™ iCycler, iQ5™ iCycler, CFX96™ (Bio-Rad, USA)
  • Str, Fla, Uni
  • DT96, DTLite (DNA-Technology, Russia)
  • Str, Fla, Uni
  • Rotor-Gene® 3000, Rotor-Gene® 6000 and Rotor-Gene® Q (Qiagen, Germany)
  • Uni, Fla
  • Eco48, (PCRMax, GB)
  • Fla

Our kits can be used with other real-time cyclers from companies ABI, ROCHE, Agilent etc. Please read the IFU properly, and ask us for recommendations for the settings.