RealLine KRAS Detect

RUO

Product name Cat.No. Packsize
RealLine KRAS Detect, Kit for Real Time PCR
[PDS]
MED20401 12 Tests
RealLine KRAS Detect, Kit for Real Time PCR
[PDS]
MED20410 36 Tests

Colorectal cancer (mCRC) is the fourth most common cancer among men and women. About 40% of mCRC patients have mutations of the KRAS gene in the tumor. Tumors with KRAS mutations in codons 12 or 13 are resistant to anti-EGFR therapy. Accordingly, anti-EGFR therapy of mCRC using Erbitux® or Vectibix® is recommended only for tumors without such mutations.

Mutant codon – Nucleotides

  • G12C – TGT
  • G12S – AGT
  • G12R – CGT
  • G12V – GTT
  • G12D – GAT
  • G12A – GCT
  • G13D – GAC

Applications

The RealLine KRAS Detect kit is designed for the detection of 7 mutations in the codons 12 and 13 of the kRas gene by real-time PCR.

Indications for testing

Targeted therapy of metastatic colorectal cancer (mCRC).

Sample Material: DNA extracted from

  • fresh tissue
  • fresh-frozen tissue
  • formalin fixed paraffin embedded (FFPE) tissue

Real-Time PCR Detection Channels

  • FAM (495-520 nm)
  • KRAS mutations and Positive Control
  • ROX (575-602 nm)
  • Internal Control IC

Validated Cyclers

  • RealLine Cycler 96 and RealLine Cycler 48 (BIORON Diagnostics GmbH)
  • Str, Fla, Uni
  • iQ™ iCycler, iQ5™ iCycler, CFX96™ (Bio-Rad, USA)
  • Str, Fla, Uni
  • DT96, DTLite (DNA-Technology, Russia)
  • Str, Fla, Uni

Our kits can be used with other real-time cyclers from companies ABI, ROCHE, Agilent, Qiagen etc. Please read the IFU properly, and ask us for recommendations for the settings.