RealLine EGFR Detect


Product name Cat.No. Packsize
RealLine EGFR Detect 7R, Kit for Real Time PCR
MED21811 12 Tests
RealLine EGFR Detect 7R, Kit for Real Time PCR
MED21812 36 Tests

Non-small cell lung cancer (NSCLC) is the most common type of the lung cancer. Activating somatic mutations within the tyrosin kinase (TK) domain in the epidermal growth factor receptor (EGFR) protein are associated with sensitivity of tumors to EGFR-TK inhibitors. Most of these mutations (~90%) occur as either in-frame microdeletions that affect codons 746-750 in exon 19 (del19), or a missense mutation L858R in exon 21 of the EGFR gene. In addition rare activating mutations (L861Q, G719S/A/C, S768I and others) make up 10 – 15 % of cases with mutant EGFR. Apart from that mutation T790M occurs in ~50 % of cases of aquired resistance to first generation EGFR-TK inhibitors. The European Society for Medical Oncology (ESMO) and American Society of Clinical Oncology (ASCO) recommend an EGFR mutation testing for selection of NSCLC patients who could benefit from anti-EGFR therapy.


The RealLine EGFR 7R Kit is detecting the mutations:

deletions and complex mutations in exon 19
G719X – detected but not genotyped G719S, G719A, G719C

Indications for testing

Patients with tumors harboring either of these mutant forms of EGFR are extremely sensitive to the selective EGFR tyrosine kinase (TK) inhibitors gefitinib (IRESSA®) or erlotinib (TARCEVA®).

Sample Material: DNA extracted from

  • fresh tissue
  • fresh-frozen tissue
  • formalin fixed paraffin embedded (FFPE) tissue

Real-Time PCR Detection Channels

  • FAM (495-520 nm)
  • EGFR Mutation and Control Mix
  • ROX (575-602 nm)
  • Internal Control IC

Validated Cyclers

  • RealLine Cycler 96 and RealLine Cycler 48 (BIORON Diagnostics GmbH)
  • Str, Fla, Uni
  • iQ™ iCycler, iQ5™ iCycler, CFX96™ (Bio-Rad, USA)
  • Str, Fla, Uni
  • DT96, DTLite (DNA-Technology, Russia)
  • Str, Fla, Uni

Our kits can be used with other real-time cyclers from companies ABI, ROCHE, Agilent, Qiagen etc. Please read the IFU properly, and ask us for recommendations for the settings.