RealLine BRAF Detect V600E

Product name Cat.No. Packsize
RealLine BRAF Detect V600E, CE - IVD
[PDS]
MED20701 12 Tests
RealLine BRAF Detect V600E, CE - IVD
[PDS]
MED20710 36 Tests
In vitro diagnosticum

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BRAF, a serine-threonine kinase, is one members of the RAF protein kinase family. Activating mutations in BRAF were described in cases of melanoma, papillary thyroid cancer, testicular, ovarian and colon cancers. Most common (~80%) of these somatic mutations is a missense mutation T1799A within the kinase domain of the BRAF that leads to change of Valine (Val) to Glutamic acid (Glu) at position 600 (V600E).
The selective inhibitor of BRAF – Zelboraf (Vemurafenib/PLX4032) (Roche) was designed for the target therapy of melanoma which harbor BRAF-V600E mutation. Other compounds against tumors that carry the mutation BRAF-V600E are in clinical studies.

Applications

The RealLine BRAF kits are designed to detect missense mutation at codon 600 of the BRAF gene which is associated with the sensitivity to the therapy with BRAF inhibitors. Also mutations of GTG to GAA, GAT, and AAG are detected by allele specific real time PCR.

Indications for testing

The kit is designed to detect missense mutation GTG→GAG at Codon 600 of the BRAF gene which is associated with the sensitivity to the therapy with BRAF inhibitors.

Sample Material: DNA extracted from

  • fresh tissue
  • fresh-frozen tissue
  • formalin fixed paraffin embedded (FFPE)tissue

Real-Time PCR Detection Channels

  • FAM (495-520 nm): BRAF-V600E mutation detection and Positive Control
  • ROX (575-602 nm): Internal Control IC

Validated Cyclers

  • RealLine Cycler 96 and RealLine Cycler 48 (BIORON Diagnostics GmbH)
  • Str, Fla, Uni
  • iQ™ iCycler, iQ5™ iCycler, CFX96™ (Bio-Rad, USA)
  • Str, Fla, Uni
  • DT96, DTLite (DNA-Technology, Russia)
  • Str, Fla, Uni

Our kits can be used with other real-time cyclers from companies ABI, ROCHE, Agilent, Qiagen etc. Please read the IFU properly, and ask us for recommendations for the settings.